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COMPARATIVE STUDY OF THE EFFECT OF SALIVA SPECIMEN AND PLAQUE SPECIMEN ON THE CORRELATION BETWEEN DENTAL CARIES EXPERIENCE AND DENTAL CARIES ACTIVITY TEST SCORE WITH ACIDIC HIGH-SUCROSE LIQUID MEDIUM IN PRESCHOOL CHILDREN

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Abstract


The purpose of this study was to compare the effect of saliva specimen and plaque specimen on the relationship between dental caries experience and dental caries activity test score by acid high-sucrose liquid medium in preschool children. The caries experience and caries activity of 270 preschool children were examined. Caries activity was measured by dental plaque and the saliva. The medium was composed of sucrose 30%, beef extract 0.1%,¢¥NaC1 0.2%, bromocresol green 0.01%. The pH of medium was adjusted to pH 5.4 by lactic acid solution. 5ml of medium was poured into capped test tubes which were then autoclaved. The plaque specimen was sampled from the subject¢¥s upper buccal tooth surfaces by a cotton applicator and then it was directly put into the tube. The saliva specimen was dropped directly into the test tube. The media were incubated at 37C for 96 hrs. Every 24 hours pH was readed by observing the color change of pH indicator bromocresol green in the medium with the color standard. 3 or 4 day¢¥s pHs were converted to scores and then added together. pH drop of plaque specimen was faster and greater than saliva ,specimen.. The correlation between caries experience and

plaque specimen score was higher than that of caries experience and saliva specimen score. Therefore, plaque seemed to be more appropriate as the specimen of the caries activity test using acid high-sucrose medium than saliva.

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